In vitro and in vivo study on the anticancer effects of anethole-loaded bovine serum albumin nanoparticles surface decorated with chitosan and folic acid

Abstract Anethole (Ant) is a herbal compound with unique properties, which limited in its clinical use due to low solubility aqueous solutions. Therefore, this study, albumin nanocarrier modified chitosan-folate was used transfer Ant cancer cells and anticancer effects were evaluated. First, loaded on nanoparticles by desolvation method then the surface of covered chitosan bound folate. After characterization, amount loading measured absorption toxicity breast cell lines, colon, normal evaluated MTT method. The real-time QPCR investigate expression changes apoptosis-related genes treated compared control cells, finally, antitumor mouse model carrying cancer. results investigation showed presence dimensions 252 nm, dispersion index 0.28 mV, charge 27.14 are trapped about 88% ATL. effect shown breast, respectively. In addition, examination gene profile under an increase BAX caspase-3 -9 along decrease Bcl-2 gene, confirms activation internal pathway apoptosis. volume tumors apoptotic areas tissue sections confirmed vivo model. inhibition percentage free concentration 25 50 mg/kg/tumor reported as 36.9%, 56.6%, 64.9%, respectively, during 15 days treatment. These effectiveness formulation inhibiting both vitro vivo.